FASTA DNA reverse complement
Reverse complement IUPAC DNA sequences in a FASTA file while retaining their headers.
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Multiple files welcome · processed one at a time
Select a tool to see its supported formats and conversion limits. Large files may exceed your browser’s memory.
Before processing large files
On phones and tablets, start with files under 20 MiB and keep this tab in the foreground. Video, OCR and 3D operations can use several times the input size in memory. Desktop browsers generally have more room, but the 128 MiB job limit is not a guarantee that every file will fit. Smaller batches reduce memory pressure.
Supported input formats
- FASTA biological sequences (.fasta)
- FASTA biological sequences (.fa)
- FASTA nucleotide sequences (.fna)
- FASTA coding nucleotide sequences (.ffn)
- FASTA amino acid sequences (.faa)
- FASTA noncoding RNA sequences (.frn)
Output format
FASTA biological sequences (.fasta)
Support applies to this conversion direction. Check the limits below for features that may change or be discarded.
Compare all supported formats and conversion limits →How to use FASTA DNA reverse complement
- Add your supported file using the chooser or drop zone.
- Review the settings and conversion limits before processing.
- Select Convert files, then download the result.
What to expect
- UTF-8, uncompressed files only, maximum 64 MiB, 100,000 records and 20 million sequence symbols. No alignment or biological interpretation.
- DNA IUPAC symbols only; RNA U, gaps, stop symbols and protein-only symbols are rejected. Case is preserved.
Engine: Local sequence parser. The engine may need a first-use download. Your file stays in this browser session.Check conversion engines and offline requirements.
About this conversion
Is fasta dna reverse complement free?
Yes. This tool runs on your device without an account or a conversion fee.
Are files uploaded during conversion?
File processing happens locally in your browser. Engines may download their code on first use; your input files are not sent to a conversion server. Read how file privacy works.
What if conversion fails?
Check that the file matches a supported input, review the engine’s limitations and try a smaller representative file. If the engine cannot load, check its engine assets and browser requirements. The app reports failures without switching to a remote converter.
Continue with your output
These tools accept this tool’s output format. Each step has its own settings and fidelity limits.
- Inspect FASTA sequences
Summarize FASTA record lengths and sequence symbol counts locally.
- FASTA to CSV
Export FASTA identifiers, descriptions and sequence strings to CSV.
- FASTA to JSON
Export FASTA identifiers, descriptions and sequence strings to JSON.
- Create TAR.GZ
Bundle selected files into a gzip-compressed USTAR archive.
- Create ZIP
Bundle the selected files into one compressed ZIP archive.
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- Inspect FASTQ reads and quality
Inspect FASTQ read lengths and explicit Phred+33 quality statistics in your browser.
- FASTQ to FASTA
Extract FASTQ read headers and sequences as FASTA, discarding quality scores.
- FASTQ to CSV
Export FASTQ headers, sequences and original quality strings to CSV.